A buffer is a solution that resists changes in pH when acid or base is added to it. Buffers are typically made by mixing a weak acid or base with its conjugate base or acid, respectively. This allows the buffer solution to neutralize small amounts of added acid or base while maintaining a relatively stable pH.
Buffers are commonly used in biological and chemical applications where maintaining a specific pH is important, such as in cell culture media, enzyme assays, and biochemical reactions. They are also used in many laboratory experiments and applications to prevent drastic changes in pH that can affect the outcome of the experiment.
Buffers can be prepared with a specific pH range by adjusting the concentrations of the weak acid and its conjugate base, or weak base and its conjugate acid. Common buffer systems include phosphate buffers, acetate buffers, and Tris buffers.
Overall, buffers play a crucial role in maintaining a stable pH in various applications and experiments, making them an essential component in many scientific processes.
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